M-CSF - Mouse M-CSF protein

产品概述

产品名称：Mouse M‑CSF protein  |  货号：PRP1311

本品为重组小鼠巨噬细胞集落刺激因子（M‑CSF），适用于细胞培养中的巨噬细胞或单核细胞系功能研究。

关键参数

别名	CSF‑1、M‑CSF、Lanimostim、CSF1
基因 ID	1436
蛋白 ID	P07141‑1
物种	小鼠
氨基酸长度	241 aa
预测分子量	27.4 kDa
表达宿主	大肠杆菌（E. coli）
纯度	> 99 %（SDS‑PAGE 检测）
活性测定	通过诱导 M‑NFS‑60 细胞增殖，ED₅₀ = 0.05417 ng/mL
内毒素水平	≤ 1.0 EU/µg（LAL 法）
产品形态	冻干粉
复溶缓冲液	20 mM Tris, 150 mM NaCl, pH 7.4（无菌）

功能与应用背景

M‑CSF（亦称 CSF‑1）在造血系统中调控单核/巨噬细胞系的存活、分化与增殖。它通过与 CSF‑1 受体结合，促进单核细胞向巨噬细胞转化，对巨噬细胞的功能成熟具有关键作用。该因子在胎盘发育、炎症模型及体外巨噬细胞培养中均有广泛使用价值。

适用细胞系

• M‑NFS‑60（活性检测）
• RAW 264.7、J774 等小鼠巨噬细胞系
• 单核细胞分化模型（如 THP‑1 转化为巨噬细胞）

使用说明

1. 凍乾粉使用前请先离心去除可能的沉淀。
2. 推荐使用配套的 Reconstitution Buffer（20 mM Tris, 150 mM NaCl, pH 7.4）复溶，浓度不低于 100 µg/mL。
3. 复溶后可根据实验需求进行梯度稀释；建议使用低吸附离心管，以减少蛋白吸附。
4. 在细胞培养基中加入 10–100 ng/mL（或根据文献/实验设定）以刺激巨噬细胞增殖或分化。

保存与稳定性

• 冻干粉：-20 ℃以下、干燥、避光保存。
• 复溶后：4 ℃保存 2–7 天；长期使用请返回 -20 ℃。
• 为降低吸附与聚集，建议在复溶液中加入 0.1 % 载体蛋白（HSA 或 BSA），并分装保存，避免反复冻融。

运输与包装

采用蓝色干冰或冰袋（蓝冰）保温运输，确保全程冷链。

法规声明 & 警告

本品仅限科研使用，不得用于人类诊断或临床治疗。使用时请遵守所在地区的专利及许可规定；如因使用本产品产生的专利侵权或其他合规问题，供应方概不负责。

订购信息

规格	单价 (人民币)	库存状态	交付时间
5 µg	588	现货	次日发货
20 µg	1498	现货	次日发货
100 µg	2898	请咨询	请咨询
1 mg	请咨询	请咨询	请咨询

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注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Product Name: Mouse M‑CSF protein | Catalog No.: PRP1311

This product is recombinant mouse macrophage colony‑stimulating factor (M‑CSF), appropriate for functional studies of macrophage or monocyte cell lines in cell culture.

Key Parameters

Alias	CSF‑1, M‑CSF, Lanimostim, CSF1
Gene ID	1436
Protein ID	P07141‑1
Species	Mouse
Amino‑acid Length	241 aa
Predicted Molecular Weight	27.4 kDa
Expression Host	Escherichia coli
Purity	> 99 % (SDS‑PAGE analysis)
Activity Assay	Induction of M‑NFS‑60 cell proliferation, ED₅₀ = 0.05417 ng/mL
Endotoxin Level	≤ 1.0 EU/µg (LAL assay)
Product Form	Lyophilized powder
Reconstitution Buffer	20 mM Tris, 150 mM NaCl, pH 7.4 (sterile)

Function and Application Background

M‑CSF (also known as CSF‑1) regulates survival, differentiation, and proliferation of monocyte/macrophage lineages within the hematopoietic system. By binding to the CSF‑1 receptor, it promotes monocyte‑to‑macrophage transition and is essential for functional maturation of macrophages. This factor is widely used in placental development studies, inflammation models, and in vitro macrophage cultures.

Applicable Cell Lines

• M‑NFS‑60 (activity assay)
• RAW 264.7, J774 and other mouse macrophage cell lines
• Monocyte differentiation models (e.g., THP‑1 differentiation into macrophages)

Usage Instructions

1. Before reconstitution, centrifuge the lyophilized powder to remove any possible precipitate.
2. Reconstitute with the supplied Reconstitution Buffer (20 mM Tris, 150 mM NaCl, pH 7.4) to a concentration not lower than 100 µg/mL.
3. After reconstitution, perform serial dilutions as required for your experiment; use low‑binding centrifuge tubes to minimize protein adsorption.
4. Add 10–100 ng/mL to cell culture medium (or as dictated by literature/experimental design) to stimulate macrophage proliferation or differentiation.

Storage and Stability

• Lyophilized powder: store at ≤ ‑20 °C, dry, protected from light.
• Reconstituted solution: store at 4 °C for 2–7 days; for longer term, return to ‑20 °C.
• To reduce adsorption and aggregation, add 0.1 % carrier protein (HSA or BSA) to the reconstitution solution, aliquot, and avoid repeated freeze‑thaw cycles.

Transport and Packaging

Shipped with dry ice or insulated gel packs (blue ice) to ensure a continuous cold chain.

Regulatory Statement & Warning

This product is intended for research use only and must not be used for human diagnostic or therapeutic applications. Users must comply with local patent and licensing regulations; the supplier assumes no responsibility for patent infringement or other compliance issues arising from product use.

Ordering Information

Specification	Unit Price (CNY)	Stock Status	Delivery Time
5 µg	588	In Stock	Next‑day shipment
20 µg	1498	In Stock	Next‑day shipment
100 µg	2898	Please inquire	Please inquire
1 mg	Please inquire	Please inquire	Please inquire

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Co. for the most up‑to‑date details.