CNTF - Rat CNTF Protein

重组大鼠睫状神经营养因子 (CNTF) 蛋白

货号：PRP1222

英文名称：Rat CNTF Protein

产品概述

本品为高纯度、无标签的重组大鼠睫状神经营养因子 (CNTF) 蛋白。该蛋白在大肠杆菌系统中表达，其氨基酸序列来源于大鼠源CNTF (P20294, Met 1-Met 200)。作为一种重要的神经营养因子，CNTF在神经科学研究领域，特别是神经元存活、分化及损伤修复等研究中具有关键价值。

产品特性

• 高纯度：经SDS-PAGE检测，纯度大于97%，确保实验结果的可靠性。
• 高生物活性：通过TF-1细胞增殖实验测定，其ED50达到0.02748 ng/mL，表明具有卓越的生物效能。
• 严格的内毒素控制：采用LAL法检测，每1μg蛋白质中内毒素(EU)含量小于1.0，有效降低对细胞实验的潜在干扰。
• 无动物源成分：本品在大肠杆菌中表达，生产过程中无动物源成分，进一步提升了产品的安全性。

技术参数

种属来源：	大鼠 (Rat)
表达宿主：	大肠杆菌
蛋白长度：	200个氨基酸
计算分子量：	22.9 kDa
SDS-PAGE表观分子量：	约24 kDa (还原条件)
产品形式：	液态，溶于无菌的20 mM Tris, 50 mM NaCl, pH 8.0缓冲液
蛋白序列：	UniProt登录号：P20294

生物活性验证

产品的生物活性通过其诱导TF-1细胞增殖的能力进行严格测定。实测ED50值为0.02748 ng/mL，证明了其强大的信号通路激活能力，可直接用于各类基于细胞的功能性实验。

应用领域

• 神经元细胞体外培养与存活研究
• 神经干细胞分化诱导
• 神经系统疾病模型建立及相关机制研究
• 少突胶质细胞功能研究

保存与运输

• 保存建议：建议长期保存于-20℃以下干燥环境。短期（2-7天）可置于4℃。为最大限度保持蛋白稳定性，建议添加载体蛋白（如0.1% HSA或BSA）后分装保存，避免反复冻融。
• 运输条件：常温运输。

使用说明

客户可根据具体实验需求，使用适当的细胞培养液或缓冲液对本产品进行进一步稀释。首次使用时，建议进行梯度稀释以确定最佳工作浓度。

规格与定价

规格	价格 (人民币)	货期
5 μg	588	现货（次日发货）
20 μg	1498	现货（次日发货）
100 μg	3998	现货（次日发货）
1 mg	请咨询	请咨询

警告：本产品仅供科学研究使用，不适用于人类或临床诊断。使用者应确保其应用不违反任何专利或许可证规定。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Rat Ciliary Neurotrophic Factor (CNTF) Protein

Catalog No.: PRP1222

English Name: Rat CNTF Protein

Product Overview

This product is a high-purity, tag‑free recombinant rat Ciliary Neurotrophic Factor (CNTF) protein. The protein is expressed in an Escherichia coli system, and its amino‑acid sequence is derived from rat CNTF (P20294, Met 1‑Met 200). As a pivotal neurotrophic factor, CNTF holds critical value in neuroscience research, especially in studies of neuronal survival, differentiation, and injury repair.

Product Characteristics

• High Purity: SDS‑PAGE analysis shows purity > 97%, ensuring reliable experimental outcomes.
• High Biological Activity: Determined by TF‑1 cell proliferation assay, with an EC50 of 0.02748 ng/mL, indicating superior bioefficacy.
• Stringent Endotoxin Control: Measured by LAL assay; endotoxin (EU) content < 1.0 per µg protein, minimizing potential interference in cell assays.
• Animal‑Component Free: Expressed in E. coli with no animal‑derived reagents used during production, further enhancing product safety.

Technical Specifications

Source Species:	Rat
Expression Host:	Escherichia coli
Protein Length:	200 amino acids
Calculated Molecular Weight:	22.9 kDa
SDS‑PAGE Apparent MW:	~24 kDa (reducing conditions)
Product Form:	Liquid, dissolved in sterile 20 mM Tris, 50 mM NaCl, pH 8.0 buffer
Protein Sequence:	UniProt accession: P20294

Bioactivity Validation

The bioactivity of the product was rigorously quantified by its ability to induce TF‑1 cell proliferation. The measured EC50 is 0.02748 ng/mL, confirming potent pathway activation and suitability for a broad range of cell‑based functional assays.

Applications

• Neuronal primary culture and survival studies
• Neural stem cell differentiation induction
• Establishment of neurodegenerative disease models and mechanistic investigations
• Oligodendrocyte functional studies

Storage and Shipping

• Storage Recommendation: Store long‑term at ≤ ‑20 °C in a dry environment. For short‑term storage (2–7 days) keep at 4 °C. To maximize stability, aliquot with a carrier protein (e.g., 0.1 % HSA or BSA) and avoid repeated freeze‑thaw cycles.
• Shipping Conditions: Ambient temperature shipping.

Usage Instructions

Customers may dilute the product in appropriate cell culture media or buffer according to experimental requirements. For first‑time use, perform a serial dilution to determine the optimal working concentration.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock (next‑day shipment)
20 µg	1498	In stock (next‑day shipment)
100 µg	3998	In stock (next‑day shipment)
1 mg	Please inquire	Please inquire

Warning: This product is intended for scientific research use only and is not suitable for human or clinical diagnostic applications. Users must ensure that its use does not infringe any patents or licensing agreements.

Note: The above information is subject to change due to product optimization or other factors; please contact Yakein Co. for the most current details.