IFN-γ - Rat IFN-γ protein

重组大鼠干扰素-γ (IFN-γ) 蛋白 (无标签)

货号: PRP1214

产品类型: 重组细胞因子

种属来源: 大鼠

表达宿主: 大肠杆菌表达系统

产品描述

重组大鼠干扰素-γ (Rat IFN-γ protein) 是通过大肠杆菌表达系统制备的高纯度蛋白质。该重组蛋白的氨基酸序列来源于大鼠 IFN-γ (UniProt ID: P01581)，由 Glu23 至 Cys156 的片段构成，分子量为 14.8 kDa，以冻干粉形式提供，未融合任何标签，更贴近天然蛋白结构，适用于对标签敏感的研究场景。

关键特性

• 高纯度：经 SDS-PAGE 检测，纯度 ≥ 95%
• 低内毒素：LAL 法检测显示，每 1 μg 蛋白质中内毒素含量 < 1.0 EU
• 无标签干扰：未添加任何融合标签，减少对蛋白功能及下游实验的潜在影响
• 冻干形态：冻干缓冲液为无菌 150mM NaCl，20mM Tris，pH 7.0 溶液，便于储存与复溶

技术参数

• 蛋白长度：134 个氨基酸
• 预测分子量：14.8 kDa
• 表观分子量（SDS-PAGE, 还原条件）：约 14.8 kDa
• 活性状态：未提供活性数据（Null）

应用建议

该产品可用于大鼠来源的细胞培养体系，在免疫应答、巨噬细胞活化、抗病毒或抗肿瘤机制等研究中作为体外刺激因子使用。建议复溶时使用推荐复溶缓冲液，浓度不低于 100 µg/mL，后续可根据实验体系进一步稀释。

保存与运输

• 保存条件：冻干粉应在 -20℃ 以下干燥环境中保存。复溶后可在 4℃ 稳定保存 2–7 天，长期保存需置于 -20℃ 以下，建议添加载体蛋白（如 0.1% HSA 或 BSA）并分装，以避免反复冻融。
• 运输条件：常温运输

背景信息

干扰素-γ (IFN-γ) 属于 II 型干扰素家族，是一种重要的免疫调节细胞因子，主要由活化的 T 细胞和 NK 细胞分泌。其在激活巨噬细胞、调节 Th1/Th2 平衡、抑制病毒复制及抗肿瘤免疫中发挥核心作用。IFN-γ 功能的异常表达也与多种自身免疫性疾病及慢性炎症相关。

规格与价格

规格	价格（人民币）	货期
20 μg	588	现货，次日发货
100 μg	1498	现货，次日发货
500 μg	4698	现货，次日发货
1 mg	请咨询	请咨询

使用注意：本品仅供科研使用，不适用于临床诊断、治疗或其他人类用途。

基因别名： IFNG, IFG, IFI, IFN-gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF

蛋白序列信息： UniProt: P01581

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Rat Interferon‑γ (IFN‑γ) Protein (Tag‑free)

Product No.: PRP1214

Product Type: Recombinant cytokine

Species Origin: Rat

Expression Host: Escherichia coli expression system

Product Description

Recombinant rat interferon‑γ (Rat IFN‑γ protein) is a high‑purity protein prepared using an E. coli expression system. The amino‑acid sequence is derived from rat IFN‑γ (UniProt ID: P01581), comprising the fragment from Glu23 to Cys156, with a molecular weight of 14.8 kDa. It is supplied as a lyophilized powder, without any fusion tags, more closely resembling the native protein structure and suitable for tag‑sensitive applications.

Key Features

• High purity: SDS‑PAGE analysis shows purity ≥ 95 %
• Low endotoxin: LAL assay indicates endotoxin < 1.0 EU per µg protein
• No tag interference: No fusion tags are added, minimizing potential impact on protein function and downstream assays
• Lyophilized format: The lyophilization buffer is sterile 150 mM NaCl, 20 mM Tris, pH 7.0 solution, facilitating storage and re‑solubilization

Technical Parameters

• Protein length: 134 amino acids
• Theoretical molecular weight: 14.8 kDa
• Apparent molecular weight (SDS‑PAGE, reducing conditions): ~14.8 kDa
• Activity status: Activity data not provided (Null)

Suggested Applications

This product can be used in rat‑derived cell culture systems as an in‑vitro stimulant in studies of immune responses, macrophage activation, antiviral or antitumor mechanisms. It is recommended to re‑constitute with the suggested buffer at a concentration ≥ 100 µg/mL, followed by dilution according to the specific assay requirements.

Storage and Shipping

• Storage conditions: Lyophilized powder should be stored at ≤ ‑20 °C in a dry environment. After re‑constitution, it is stable at 4 °C for 2–7 days; for long‑term storage keep at ≤ ‑20 °C, preferably with a carrier protein (e.g., 0.1 % HSA or BSA) and aliquoted to avoid repeated freeze‑thaw cycles.
• Shipping conditions: Ambient temperature

Background Information

Interferon‑γ (IFN‑γ) belongs to the type II interferon family and is a pivotal immunoregulatory cytokine primarily secreted by activated T cells and NK cells. It plays central roles in macrophage activation, Th1/Th2 balance regulation, inhibition of viral replication, and antitumor immunity. Aberrant IFN‑γ expression is associated with various autoimmune diseases and chronic inflammatory conditions.

Specifications and Pricing

Specification	Price (CNY)	Lead Time
20 µg	588	In stock, next‑day shipment
100 µg	1498	In stock, next‑day shipment
500 µg	4698	In stock, next‑day shipment
1 mg	Please inquire	Please inquire

Usage note: This product is for research use only and is not intended for clinical diagnosis, therapy, or any other human applications.

Gene aliases: IFNG, IFG, IFI, IFN‑gamma, Immune Interferon, Type II Interferon, T Cell Interferon, MAF

Protein sequence information: UniProt: P01581

Note: The above information may change due to product optimization or upgrades; please contact Yakeyin Co. for the most current details.