IL-33 - Human IL-33 protein

IL-33 - Human IL-33 protein (货号: PRP1018)

产品概述

核心产品类型：细胞因子（细胞因子及蛋白）
适用范围：细胞类研究 → 细胞培养 → 细胞因子功能分析

关键特性

• 重组人白细胞介素‑33（IL‑33），氨基酸序列来源于人源 IL‑33 亚型（O95760）Ser112‑Thr270，长度 160 aa，预测分子量 18.1 kDa。
• 表达宿主：大肠杆菌。
• 纯度：> 98 %，通过 SDS‑PAGE 检测。
• 无标签（native）蛋白，活性标记为 Null，适用于需要原始结构的实验。
• LAL 法检测：每 1 µg 蛋白质中的 EU 含量 < 0.01，适合对内毒素敏感的细胞培养体系。

产品形态 & 配套缓冲液

本品以冻干粉形式提供，配套的重组缓冲液为无菌 20 mM Tris, 50 mM NaCl, 1 mM DTT, 1 mM EDTA，pH 5.0。

推荐使用配套缓冲液复溶，重组人 IL‑33 的浓度不低于 100 µg/mL，复溶后可根据实验需求进行稀释。

规格与报价（均为现货，次日发货）

规格	单价（人民币）	可用库存
5 µg	588	现货
20 µg	1498	现货
100 µg	4516	现货
1 mg	请咨询	现货

使用建议

1. 打开包装后请先进行离心除去可能的沉淀。
2. 使用推荐缓冲液复溶，确保最终浓度 ≥ 100 µg/mL。
3. 如需长期保存，建议在 4 °C 条件下保存 2–7 天；更长时间请保持在 -20 °C 以下。
4. 为降低冻融对活性的影响，可在复溶后加入 0.1 % HSA 或 BSA 作为载体蛋白，并分装存储。

保存与运输

• 冻干粉：建议在 -20 °C 以下的干燥环境中长期保存。
• 已复溶产品：4 °C 可保存 2–7 天，长期保存请回冻至 -20 °C。
• 运输方式：使用蓝色冰袋（冰袋运输），确保全程低温。

背景信息（便于方案设计）

IL‑33 属于 IL‑1 家族的促炎蛋白，亦被称为 DVS27、NF‑HEV。它在扁桃体、派尔集合淋巴结及高内皮微静脉内皮细胞中高表达，兼具细胞因子和核因子双重功能。IL‑33 在 2 型免疫应答、蠕虫感染及变态反应性疾病中发挥关键作用，常用于探索炎症信号通路、细胞因子网络以及细胞内转录调控机制。

技术参数摘要

基因 ID	90865
蛋白质 ID	O95760
别名	C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, Interleukin‑33, IL33
检测指标	Null
分子量	约 18 kDa
序列链接	UniProt O95760

重要声明

本产品仅供科学研究使用，不适用于人类或临床诊断。使用本产品的任何行为均应遵守当地法规和专利许可。对于因使用本产品可能引发的专利侵权或其他违规行为，供应商不承担任何责任。

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如需进一步技术支持或批量采购，请联系我们的商务团队。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

IL-33 - Human IL-33 Protein (Cat#: PRP1018)

Product Overview

Core Product Type: Cytokine (Cytokines & Proteins)
Application Area: Cell-based Research → Cell Culture → Cytokine Functional Analysis

Key Features

• Recombinant Human Interleukin-33 (IL-33), amino acid sequence derived from human IL-33 isoform (O95760) Ser112-Thr270, length 160 aa, predicted molecular weight 18.1 kDa.
• Expression Host: E. coli.
• Purity: > 98%, as determined by SDS-PAGE.
• Tag-free (native) protein, activity designation: Null, suitable for experiments requiring the native structure.
• LAL endotoxin assay: < 0.01 EU per 1 µg of protein, suitable for cell culture systems sensitive to endotoxins.

Product Form & Reconstitution Buffer

This product is supplied as a lyophilized powder. The accompanying recombinant buffer is a sterile solution containing 20 mM Tris, 50 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 5.0.

Reconstitution using the provided buffer is recommended. The reconstituted Human IL-33 concentration is ≥ 100 µg/mL. It can be diluted according to experimental requirements after reconstitution.

Specifications & Pricing (In Stock, Shipped Next Day)

Specification	Unit Price (RMB)	Available Stock
5 µg	588	In Stock
20 µg	1498	In Stock
100 µg	4516	In Stock
1 mg	Please Inquire	In Stock

Usage Suggestions

1. Centrifuge the vial briefly upon opening to pellet any potential precipitate.
2. Reconstitute using the recommended buffer to ensure a final concentration ≥ 100 µg/mL.
3. For short-term storage, store at 4°C for 2-7 days; for long-term storage, maintain at -20°C or below.
4. To minimize activity loss from freeze-thaw cycles, consider adding 0.1% HSA or BSA as a carrier protein after reconstitution and aliquot before storage.

Storage & Shipping

• Lyophilized Powder: Recommended for long-term storage in a dry environment at -20°C or below.
• Reconstituted Product: Store at 4°C for up to 2-7 days; for long-term storage, re-freeze at -20°C.
• Shipping Method: Shipped with blue ice packs (gel packs) to ensure a consistent cold chain.

Background Information (For Experimental Design)

IL-33 is a pro-inflammatory protein belonging to the IL-1 family, also known as DVS27 or NF-HEV. It is highly expressed in tonsils, Peyer's patches, and high endothelial venule endothelial cells, functioning both as a cytokine and a nuclear factor. IL-33 plays a key role in type 2 immune responses, helminth infections, and allergic diseases. It is commonly used to investigate inflammatory signaling pathways, cytokine networks, and intracellular transcriptional regulation mechanisms.

Technical Parameter Summary

Gene ID	90865
Protein ID	O95760
Aliases	C9orf26, DVS27, IL1F11, NF-HEV, NFEHEV, Interleukin-33, IL33
Activity Assay	Null
Molecular Weight	Approx. 18 kDa
Sequence Link	UniProt O95760

Important Notice

This product is intended for research use only, not for human or clinical diagnostic use. Any use of this product should comply with local regulations and patent licenses. The supplier assumes no liability for any patent infringement or other violations that may arise from the use of this product.

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For further technical support or bulk purchase inquiries, please contact our commercial team.

Note: The above information is subject to change due to factors such as product optimization and upgrades. Please consult Yakeyin Company for specific details.