Flt-3 Ligand 人源重组蛋白 细胞因子

产品概述

Human Flt-3 Ligand protein, His tag (Animal-Free) 是一款高纯度、高活性的人源重组细胞因子，专为细胞培养及相关生命科学研究设计。本品采用无动物源（Animal-Free）表达系统生产，有效降低了外源因子污染风险，为干细胞研究、免疫学及造血发育等领域提供可靠工具。

产品核心信息

• 产品名称：Flt-3 Ligand 人源重组蛋白 细胞因子
• 英文名称：Human Flt-3 Ligand protein, His tag (Animal-Free)
• 货号：PRP1062
• 种属来源：人源
• 表达宿主：大肠杆菌表达系统
• 标签类型：C端6×His标签

产品特性与质量控制

本品通过基因重组技术表达，序列来源于人源Flt-3配体（UniProt ID: P49771）的Thr27-Ala181片段，蛋白全长155个氨基酸，预测分子量为17.6 kDa。

• 纯度：> 95%（SDS-PAGE检测）
• 生物活性：经BaF3细胞增殖实验验证，ED₅₀ < 0.8 ng/mL，比活性 > 1.5 × 10⁶ IU/mg
• 内毒素控制：每1 μg蛋白质中EU含量 < 0.1（LAL法检测）

应用方向

Flt-3 Ligand是造血系统中的关键调控因子，可与其他细胞因子（如GM-CSF、IL-3、TPO等）协同作用，促进淋巴系和髓系造血祖细胞的增殖与分化。适用于：

• 造血干细胞体外扩增培养
• 免疫细胞功能研究
• 联合细胞因子诱导多能干细胞分化
• 相关信号通路机制探索

使用说明与保存条件

复溶建议：推荐使用无菌ddH₂O复溶，浓度不低于100 μg/mL。复溶后请在室温静置至少20分钟，避免振荡，以保持蛋白生物活性。

保存条件：冻干粉在-20°C下可稳定保存1年；复溶后于2-8°C可保存1周。若在含稳定剂（如0.1% BSA）的缓冲液中稀释分装，于-20°C或-80°C可保存3-6个月，建议避免反复冻融。

运输方式：冰袋（蓝冰）运输，确保产品在途稳定性。

规格与价格（人民币）

注意事项

本品仅限科研使用，不适用于临床诊断、药物开发或人体应用。使用前请务必离心处理（3000 rpm，5分钟），并严格遵守推荐操作流程。具体应用请结合实验体系进行条件优化。

别名参考：FL, FLG3L, FLT3L, 重组人Fms相关酪氨酸激酶3配体

蛋白序列信息：UniProt: P49771

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Human Flt-3 Ligand protein, His tag (Animal-Free) is a high‑purity, high‑activity human recombinant cytokine designed for cell culture and related life‑science research. The product is produced using an Animal‑Free expression system, effectively reducing the risk of exogenous factor contamination and providing a reliable tool for stem‑cell research, immunology, and hematopoietic development.

Core Product Information

• Product Name: Flt-3 Ligand Human Recombinant Protein Cytokine
• English Name: Human Flt-3 Ligand protein, His tag (Animal-Free)
• Catalog Number: PRP1062
• Species Origin: Human
• Expression Host: Escherichia coli expression system
• Tag Type: C‑terminal 6×His tag

Product Characteristics and Quality Control

This product is expressed using recombinant DNA technology; the sequence corresponds to the human Flt-3 ligand (UniProt ID: P49771) Thr27‑Ala181 fragment. The protein comprises 155 amino acids with a predicted molecular weight of 17.6 kDa.

• Purity: >95% (SDS‑PAGE)
• Biological Activity: Demonstrated in BaF3 proliferation assay, ED₅₀ < 0.8 ng/mL, specific activity > 1.5 × 10⁶ IU/mg
• Endotoxin Level: <0.1 EU per µg protein (LAL assay)

Applications

Flt-3 Ligand is a pivotal regulator in the hematopoietic system; it synergizes with other cytokines (e.g., GM‑CSF, IL‑3, TPO) to promote proliferation and differentiation of lymphoid and myeloid progenitors. Suitable for:

• Ex vivo expansion of hematopoietic stem cells
• Immunocyte functional studies
• Combined cytokine‑induced differentiation of pluripotent stem cells
• Exploration of related signaling pathway mechanisms

Usage Instructions and Storage Conditions

Reconstitution Recommendation: Reconstitute with sterile ddH₂O to a concentration ≥100 µg/mL. After reconstitution, allow the solution to stand at room temperature for at least 20 minutes, avoiding vortexing, to preserve biological activity.

Storage Conditions: The lyophilized powder is stable at –20 °C for up to 1 year; reconstituted material can be stored at 2–8 °C for 1 week. When diluted and aliquoted in a buffer containing stabilizers (e.g., 0.1 % BSA), it can be stored at –20 °C or –80 °C for 3–6 months; repeated freeze‑thaw cycles should be avoided.

Shipping Method: Shipped on ice packs (blue ice) to maintain product stability during transit.

Specifications and Pricing (CNY)

Precautions

This product is intended for research use only and is not suitable for clinical diagnostics, drug development, or human application. Prior to use, centrifuge (3,000 rpm, 5 min) and strictly follow the recommended protocol. Specific applications should be optimized within the context of your experimental system.

Synonyms: FL, FLG3L, FLT3L, recombinant human Fms‑related tyrosine kinase 3 ligand

Protein Sequence Information:UniProt: P49771

Note: The above information is subject to change due to product optimization or upgrades; please contact Yakein Co. for the most current details.