CCL3 人源重组蛋白 细胞因子

Human CCL3 Protein, His tag (Animal-Free) - PRP1925

本品为高纯度人源重组CCL3蛋白（Animal-Free），专为细胞培养及免疫学研究设计，适用于趋化性实验、炎症机制研究及相关药物筛选平台。

产品特性

• 基因来源：人源C-C基序趋化因子配体3（CCL3/MIP-1α）
• 表达系统：大肠杆菌表达系统，无动物源成分，降低外源因子风险
• 纯度：经SDS-PAGE验证，纯度 > 95%
• 内毒素控制：LAL法检测，每微克蛋白内毒素含量 < 0.1 EU
• 活性验证：可通过化学吸附人PBMC实验验证其功能活性，推荐使用浓度范围为5–50 ng/mL

产品形式与复溶建议

本品为冻干粉形式，缓冲体系为无菌50 mM Tris，150 mM NaCl，pH 8.5。推荐使用配套复溶缓冲液（Reconstitution Buffer）复溶至浓度不低于100 µg/mL，后续可根据实验体系进一步稀释。

保存与运输

• 保存条件：冻干粉可在-20°C稳定保存1年；复溶后于2–8°C可存放1周。进一步稀释建议添加稳定剂（如0.1% BSA、10% FBS、5% HSA），并分装存于-20°C或-80°C，避免反复冻融。
• 运输：全程蓝冰低温运输，保障产品稳定性。

生物学背景

CCL3（亦称巨噬细胞炎症蛋白-1α, MIP-1α或LD78α）是一种由66个氨基酸组成、分子量约8–11 kDa的趋化因子。其在急性炎症反应中发挥核心作用，可通过结合CCR1、CCR4和CCR5受体，介导白细胞趋化与活化，参与发热反应及免疫调节过程。

注意事项

使用前请离心，确保产品完全沉降。本产品仅限于科研用途，不应用于临床诊断或人体使用。使用者应自行确保实验符合相关法律法规及知识产权要求。

可选规格与价格

规格	价格（元）	货期
5 µg	588	现货，次日发货
20 µg	1498	现货，次日发货
100 µg	4539	请咨询
1 mg	请咨询	请咨询

警告：本品仅用于科学研究，不适用于人体或临床诊断。用户须自行承担使用过程中可能涉及的专利或合规责任。

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Human CCL3 Protein, His tag (Animal-Free) - PRP1925

This product is a highly purified, recombinant human CCL3 protein (Animal-Free), specifically designed for cell culture and immunology research. It is suitable for chemotaxis assays, inflammation mechanism studies, and related drug screening platforms.

Product Features

• Gene Source: Human C-C motif chemokine ligand 3 (CCL3/MIP-1α)
• Expression System: E. coli expression system, animal-free components, reducing the risk of exogenous factors
• Purity: > 95% as verified by SDS-PAGE
• Endotoxin Control: < 0.1 EU/µg protein as detected by LAL assay
• Activity Validation: Functional activity can be validated through chemotactic assays using human PBMCs; recommended working concentration range is 5–50 ng/mL

Product Form and Reconstitution Recommendation

This product is supplied as a lyophilized powder in a sterile buffer system containing 50 mM Tris, 150 mM NaCl, pH 8.5. It is recommended to reconstitute with the provided Reconstitution Buffer to a concentration not less than 100 µg/mL, and further dilution can be performed according to the experimental system.

Storage and Shipping

• Storage Conditions: The lyophilized powder is stable for 1 year when stored at -20°C; after reconstitution, it can be stored at 2–8°C for 1 week. For further dilution, adding stabilizers (e.g., 0.1% BSA, 10% FBS, 5% HSA) is recommended. Aliquot and store at -20°C or -80°C to avoid repeated freeze-thaw cycles.
• Shipping: Shipped with blue ice to ensure product stability.

Biological Background

CCL3 (also known as Macrophage Inflammatory Protein-1α, MIP-1α or LD78α) is a chemokine composed of 66 amino acids with a molecular weight of approximately 8–11 kDa. It plays a central role in acute inflammatory responses by binding to CCR1, CCR4, and CCR5 receptors, mediating leukocyte chemotaxis and activation, and participating in fever responses and immune regulation processes.

Precautions

Centrifuge the product before use to ensure complete sedimentation. This product is intended for research use only and is not for clinical diagnosis or human use. Users are responsible for ensuring that their experiments comply with relevant laws, regulations, and intellectual property requirements.

Available Specifications and Pricing

Specification	Price (CNY)	Lead Time
5 µg	588	In stock, ships next day
20 µg	1498	In stock, ships next day
100 µg	4539	Please inquire
1 mg	Please inquire	Please inquire

Warning: This product is for scientific research only and is not suitable for human use or clinical diagnosis. Users must assume responsibility for any potential patent or compliance issues related to its use.

Note: The above information is subject to change due to factors such as product optimization and upgrades. For specific details, please consult Ya Keyin Company.