FGF-8a 人源重组蛋白 细胞因子

产品概述

Human FGF-8a Protein, His tag (Animal-Free) 是一种高纯度、动物源游离的重组人源细胞因子蛋白，适用于细胞培养及相关科研应用。该产品采用大肠杆菌表达系统，经严格质控，具备优异的生物活性与稳定性，可满足各类精细实验需求。

产品详情

• 产品名称：FGF-8a 人源重组蛋白
• 英文名称：Human FGF-8a Protein, His tag (Animal-Free)
• 货号：PRP1196
• 蛋白序列：人源FGF-8a (Gln52-Arg233) (UniProt: P55075)
• 标签：C-terminal His Tag
• 表达宿主：大肠杆菌
• 纯度：> 98% (SDS-PAGE 检测)
• 分子量：22.14 kDa
• 蛋白长度：182 个氨基酸

生物活性

经3T3细胞增殖实验测定，其半数有效浓度（ED₅₀）< 2 μg/mL，显示出良好的生物学功能。

产品形式与复溶建议

本品为冻干粉形式，冻干缓冲液为无菌PBS (pH 8.0)。建议使用配套复溶缓冲液（Reconstitution Buffer）进行复溶，复溶浓度不低于 100 µg/mL，后续可根据实验需要进行稀释。

特点与优势

• 动物源游离表达系统，降低外源因子干扰
• 内毒素含量低：< 0.1 EU/μg (LAL法测定)
• 高纯度与明确分子量，便于实验标准化

保存与运输

• 保存条件：冻干粉可在 -20°C 下保存一年；复溶后于 2–8°C 可保存一周。稀释后分装保存于 -20°C 或 -80°C 可维持 3–6 个月稳定性，请避免反复冻融。
• 运输条件：蓝冰冰袋运输，保障产品活性。

背景信息

FGF-8a（Fibroblast Growth Factor-8a）是一种在胚胎发育、细胞增殖、分化及迁移中起关键作用的细胞因子。其在肢体、大脑、眼睛和耳朵的形成过程中具有重要功能。该蛋白也被称为 AIGF、HBGF-8 或 FGF8，基因ID为2253。

规格与价格

注意事项

使用前请离心。本品仅供科研使用，不适用于人体或临床诊断。使用者应自行确保不侵犯现有专利或许可协议。

更多信息可参考 UniProt 数据库：P55075

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Product Overview

Human FGF-8a Protein, His tag (Animal-Free) is a high-purity, animal-free recombinant human cytokine protein suitable for cell culture and related research applications. This product is produced using an E. coli expression system, undergoes strict quality control, and possesses excellent biological activity and stability, meeting the requirements of various sophisticated experiments.

Product Details

• Product Name: FGF-8a Human Recombinant Protein
• English Name: Human FGF-8a Protein, His tag (Animal-Free)
• Catalog Number: PRP1196
• Protein Sequence: Human FGF-8a (Gln52-Arg233) (UniProt: P55075)
• Tag: C-terminal His Tag
• Expression Host: E. coli
• Purity: > 98% (as determined by SDS-PAGE)
• Molecular Weight: 22.14 kDa
• Protein Length: 182 amino acids

Biological Activity

Determined by a 3T3 cell proliferation assay, the half-maximal effective concentration (ED₅₀) is < 2 μg/mL, demonstrating good biological function.

Product Form and Reconstitution

This product is supplied as a lyophilized powder, lyophilized from a sterile PBS buffer (pH 8.0). It is recommended to reconstitute using the accompanying Reconstitution Buffer to a concentration not less than 100 µg/mL, and it can be further diluted according to experimental needs.

Features and Advantages

• Animal-free expression system, reducing the risk of exogenous factor contamination
• Low endotoxin level: < 0.1 EU/μg (determined by LAL assay)
• High purity and well-defined molecular weight, facilitating experimental standardization

Storage and Shipping

• Storage Conditions: The lyophilized powder can be stored at -20°C for one year. After reconstitution, store at 2–8°C for up to one week. For long-term storage, aliquot the diluted solution and store at -20°C or -80°C to maintain stability for 3–6 months. Avoid repeated freeze-thaw cycles.
• Shipping Conditions: Shipped with blue ice packs to ensure product activity.

Background Information

FGF-8a (Fibroblast Growth Factor-8a) is a key cytokine involved in embryonic development, cell proliferation, differentiation, and migration. It plays important roles in the formation of limbs, brain, eyes, and ears. This protein is also known as AIGF, HBGF-8, or FGF8, with Gene ID 2253.

Specifications and Pricing

Notes

Briefly centrifuge the vial before use. This product is for research use only and is not intended for human or diagnostic use. The user is responsible for ensuring non-infringement of existing patents or license agreements.

For more information, please refer to the UniProt database: P55075

Note: The above information is subject to change due to product optimization and upgrades. Please consult Yakeyin Company for specific details.