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文献和实验mg/ml sheared salmon sperm DNA and add 50 ?l 10mg/ml E. coli tRNA (prepared from solid by adding 10 mg to 1.0 ml DDW). Store at -20°C. 4. Hybridization buffer: To make 100 µl, take 60 µL DDW and add 20 µL BSA, and 20 µL 20X SSC stock. Prepare fresh
ml of 10 mM TEAB into an Erlenmeyer flask. Add approximately 5 g of Sephadex G-50 and swirl to absorb the liquid. Suspension will settle. Store at 4°C. Prior to use, apply vacuum pressure to the flask to degas the suspension for at least 2 hours
Single Cell Gene Expression Profiling: Multiplexed Expression
into an Erlenmeyer flask. Add approximately 50 g of Sephadex G-50 and swirl to absorb the liquid. Suspension will settle. Store at 4°C. Prior to use, apply vacuum pressure to the flask to degas the suspension for at least 2 hours before pouring matrix.
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