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- Thermo Fisher
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- 2025年12月31日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
onto the top of the mineral oil overlay. Set sample in ice bucket until all samples have been prepared. Microfuge briefly to move the master mix through the mineral oil overlay. Rapidly heat the samples to 94°C and cycle 30 times through a program appropriate
experiment.Disslve in 100% EtH) 1 mM Benzamidine 5 mM Idacetamide Whatman Paper Centrifuge Bttles Funnel Squeeze bttle Several Beakers Hmgenizer (Fisher PwerGen 125) 50ml Crning tubes Ice Bucket & Ice Dunce hmgenizer (40ml Pyrex Cat N.7727
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