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文献和实验V294.PART II,Chapter 5 细胞粘附实验(英文版)
of either cells or purified ligand. (Cells or ligand willbe pre-coated on either a tissue culture dish or a cover slip, depending on themanufacturer of the flow chamber.) Work out any bubbles in the system that mayhave formed during the assembly process
V294.PART II,Chapter 2 Boyden法体外趋化试验
. Some of theimportant factors to be considered are the number of cells to load on the chamber,the pore size of the membrane, the type and concentration of the attractant,and the incubation time. Note that the ECM protein can be coated on the membrane or directly loaded
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
(PSG). Do not remove the laminin from the wells until immediately before use. Differentiation 16. Add 100 µL of NEP complete medium to each well of a poly-D-lysine/laminin-coated eight-well chamber slide (prepared
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