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文献和实验system, or interaction trap, is one of the most versatile methods available with which to identify and establish protein?protein interactions. The dual bait system is one adaptation of the classic approach. This system facilitates the simultaneous comparison
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
from the developing mouse and adult cortex. RELATED INFORMATION This protocol was adapted from the method by Polleux and Ghosh (2002) . MATERIALS Reagents Dulbecco’s phosphate-buffered saline (PBS), 1X sterile (Sigma D8537) Enzyme
Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
Basic Protocol 3: Expressing the RhoA Single‐Chain Biosensor Basic Protocol 4: Imaging the RhoA Biosensor Basic Protocol 5: Expression of Rac1 Flair, Dual‐Chain Fret Biosensor for Rac1
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