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文献和实验‐Q purified water (Millipore), or equivalent Ammonium bicarbonate 50% (v/v) acetonitrile, HPLC grade (Fisher Scientific
, label culture dishes. For 1 type of tissue received, set up 8 dishes. For 2 types of tissue received, set 6 on each; for 3 or more types of tissue, set 4 on each. Centrifuge the tissue in collagenase for 6 minutes at 150 X g (900 rpm in Sorval GLC-2B
Procedures for In Vitro Production of Bovine Embryos
tubes (05-539-5)18 g 1 ½” needles (14-826-5D)50 ml sterile centrifuge tubes (05-538-67) 60 X 15 petri dishes (08757100B) (Falcon 351007) Absorbent paper (14-206-65)Beakers 10 ml (02-540C) 50 ml (02-540G) 400 ml (02-555
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