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文献和实验Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
Microcentrifuge, preferably with a fixed‐angle rotor Vortex mixer Multidrop micro reagent dispenser (Thermo‐Fisher
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