099-062034赛默飞离心机附件Thermo Fishe

DNA Origami: Synthesis and Self‐Assembly

, J.S., Birkedal, V., Besenbacher, F., Gothelf, K.V., and Kjems, J. 2009. Self‐assembly of a nanoscale DNA box with a controllable lid. Nature 459:73‐76.    Castro, C.E

质谱的试剂用品、实验操作、仪器和样品选择方法

(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP

Western Blot Analysis by SDS

Assembly Put Green lid on (red/red and black/black) - Power Supply oTurn ON o Constant Volt - Run @ 120V ~10min (get samples through stacking gel) - Run @ 100V until marker is near bottom of gel (~1.5 hrs) Transfer Additional