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- 2025年12月31日
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
tissues once with 0.5x MBS/125 mM glycine, add fresh 0.5x MBS/125 mM glycine and incubate for 30 min at RT with horizontal rocking. Do not remove all solutions to rinse tissues; otherwise they may be broken by surface tension. (4) Rinse tissues twice
E-Z 96 X-press Plasmid Vacuum Protocol
with swinging-bucket rotor capable of 3,000 x g 2. Vacuum Manifold (Cat# Vac-03) 3. Vortex 实验步骤 1. Pick up a single colony from a fresh streaked selective plate and Inoculate 2-3 mL LB medium containing the appropriate
Gradient‐type HPLC system, preferably equipped with a PDA detector Reversed‐phase HPLC column [Waters µBondasphere C18 5 µm 300A (3.9 ×150–mm) or XBridge C18 5 µm
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