238001赛默飞摇床附件Thermo Fisher 18

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

PCR amplification of variable regions (SOE-Protocol)

sequences from the SOE-PCR are amplified using two primer mixes: univ VH (MuPD 18) and univ VL (MuPD 33). Finally, these scFv sequences contain SfiI and NotI restriction sites at their borders, so they can be cloned in the phage display vector pHEN