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- Thermo Fisher
- RBA9-18-150
- 2025年12月31日
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
block or water bath 150 mg, 300 mg, and 1 g carbograph solid phase extraction (SPE) columns (Alltech) Rotary evaporator (e
In Situ HYBRIDIZATION TO TISSUE SECTIONS
lower an 18 x 18mm coverslip from one side, evenly distributing the hyb so as to cover the entire tissue section. Avoid air bubbles! To avoid evaporation, seal the edges of the coverslip with rubber cement using a broken off Pasteur pipet
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