50155668赛默飞固定高度支架Thermo Fisher

A quick RNA mini-prep for Neurospora mycelial cultures

volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice

染色体组型分析（Karyotype Analysis）

syringes      Eagle's spinner modified media with PHA      Culture flasks      Tissue culture grade incubator at 37° C      10 µ g/ml Colcemid      Clinical centrifuge and tubes      0.075 M KCl      Absolute Methanol and glacial acetic acid (3:1 Mixture

Karyotype Analysis

and tubes 0.075 M KCl Absolute Methanol and glacial acetic acid (3:1 Mixture, prepared fresh) Dry ice Slides, coverslips and permount Alkaline solution for G-banding Saline-Citrate for G-banding Ethanol (70% and 95