75003713赛默飞离心机附件Thermo Fisher

Preparation of Tonsil Lysate

mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentr,hi) RPMI-1640 medium Tritn X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprtinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh the day f

Analys of Genomic DNA by Southern Hybridization

far outside from samples to avoid contamination or interference with sample hybridization) 4.Submerge gel in 2000 ml 1X TAE with 0.5 µg/ml ethidium bromide. (i.e. 40 ml of 50 x TAE, 100 µl of 1% ethidium bromide stock solution, dH2 O ad 2 liter

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15