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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
purity. Prolonged incubation may lead to nicking of plasmid DNA. (Store Solution II tightly capped when not in use.) 5. Add 5 ml ice-cold Buffer N3, cover, and gently mix by inverting tube several times until a flocculent white precipitate forms
Fastfilter Plasmid Midi Kit Spin Protocol
plasmid purity. Prolonged incubation may lead to nicking of plasmid DNA. (Store Solution II tightly capped when not in use.) 5. Add 1.25 ml ice-cold Buffer N3, cover, and gently mix by inverting tube several times until a flocculent white precipitate
. 3. Roll membrane(s) between mesh into tube so that prehybridization buffer will coat all surfaces. 4. Add warmed prehybridization buffer to tube and incubate at 65℃ for at least two hrs. For large tube use 25-50ml prehybridization buffer depending
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