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文献和实验Dual-Color ELISPOT Assay for the Simultaneous Detection of IL-2 and/or IFN- Secreting T Cells
, activate AEC buffer using H2 O2 (see Step 25). Anti-CD3 monoclonal antibody (mAb) (Fisher Scientific) Blocking buffer for ELISPOT assay CEF peptide pool (available from the NIH AIDS Research and Reagent Program and other commercial
Slide Sectioning Paraffin blocks- For DNA analysis: Special LCM processing schedule is followed. The water bath is cleaned using RNAse Zap™, rinsed thoroughly with triple distilled water. The water bath is filled with triple
LCM PROTOCOLS:Slide Sectioning
(Fisher) 1x Automation Buffer (Fisher) NIH protocol modified by NIEHS Thumbnail Images Procedure For Printing Thumbnails/Images From NIEHS LCM 1) Start ARC-100 software program and advance to LCM System User Selection
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