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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
-S in 500 ml 1% acetic acid(Fisher# BP103-50)Developing Solution100 mM Tris 9.5 15 ml 1M Tris 9.5100 mM NaCl 3 ml 5M NaCl5 mM MgCl2 0.75 ml 1M MgCl2up to 150ml with QTo use, add 66 ml NBT and 50 ml BCIP to 15 ml of Developing Solution.NBT (sigma N
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