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文献和实验Selection of a Platform for Mutation Detection
where it is difficult to keep track of all available options as well as their benefits and pitfalls. This unit provides an entry point for a variety of researchers who wish to analyze samples for known or novel mutations and need to determine which platform
to even slightly alkaline conditions. 3) Trypsinise the fibroblasts, spin at 400 x g for 5 mins and remove supernatant. 4) During 5 mins spin above: Prepare 12 x 35 mm plastic dishes - normally achieve 12 dishes from one flask
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
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