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文献和实验SDS-PAGE Western Blotting Protocols
-PAGE, although it does reduce the effective size of the resolving gel. 8. Add upper layer of water-saturated n-butanol to the top of the gel. 9. Let polymerize (this takes ~10-15’). 10. Invert gel to remove n-butanol. Touch with filter paper to wick off residual
Materials: Whatman 3 mm Blotting Paper nitrocellulose (Schleicher & Schuell, Amersham) or nylon membrane filter (Amersham). Paper towels (preferably C-fold "cheap-o" variety) Pyrex or Tupperware dish, glass plates and rubber stoppers A weight
Materials: Whatman 3 mm Blotting Paper nitrocellulose (Schleicher & Schuell, Amersham) or nylon membrane filter (Amersham). Paper towels (preferably C-fold "cheap-o" variety) Pyrex or Tupperware dish, glass plates and rubber
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