75006441赛默飞离心机附件Thermo Fisher

Appendix F: Centrifugation

from the vertical (such as the Sorvall SS-34 rotor or the Beckman JA-20). Figure F.1 presents a cross- sectional diagram of the Sorvall SS-34. Swinging bucket rotors (also known as horizontal rotors) have the advantage that there is usually a clean meniscus

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

(37%, Fisher, Cat# F79-500)- Formamide (Boehringer Mannheim Cat# 100 731)- Glycerol (Boehringer Mannheim Cat#100 649)- Glycine (Sigma Cat# G-4392)- Glycogen (Boeringher Mannheim Cat# 901-393)- Heparin (Sigma, Cat# H-3393)- Hepes