88881141赛默飞24x30mm可调节试管架Thermo

λ噬菌体DNA的制备方法

can be increased to 75 mM in the Elution buffer if final DNA yields are consistently low). 5. Add 0.6 ml of Elution buffer and collect the flow-through in sterile eppendorf tubes placed in a Sarstedt rack. Transfer a 10 µl aliquot into another eppendorf tube

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

超级感受态细胞的制备

of cells that receives no plasmid DNA at all. Store the tubes on ice for 30 minutes. Transfer the tubes to a rack placed in a preheated 42ºC circulating water bath. Store the tubes in the rack for exactly 90 seconds. Do not shake