03968赛默飞离心机附件（离心管）Thermo Fishe

质谱的试剂用品、实验操作、仪器和样品选择方法

(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP

Southern Hybridization Protocols

(Polyvinyl pyrrolidone) (Fisher Bioreagents) 300mg BSA (A-9647 Sigma) Top up autoclaved H2O up to 30ml (do NOT autoclave solution: BSA will coagulate!) Filter with 0.2 m . Store at -20℃ Preparation of 50% dextran sulphate (10ml) 5g Dextran sulphate 10ml

How to build a BAC library

AND ELECTROPORATION BAC LIGATION DNA should be in low melting agarose, in TAE or stored in 0.05M EDTA. Dialyze the sample in 50 ml tube at 4°C against 1 X TE, 1 X PA for 3-5hr with one change of solution. Melt agarose at 65°C for 10 minutes, transfer