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- 2025年12月31日
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文献和实验(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP
Southern Hybridization Protocols
(Polyvinyl pyrrolidone) (Fisher Bioreagents) 300mg BSA (A-9647 Sigma) Top up autoclaved H2O up to 30ml (do NOT autoclave solution: BSA will coagulate!) Filter with 0.2 m . Store at -20℃ Preparation of 50% dextran sulphate (10ml) 5g Dextran sulphate 10ml
AND ELECTROPORATION BAC LIGATION DNA should be in low melting agarose, in TAE or stored in 0.05M EDTA. Dialyze the sample in 50 ml tube at 4°C against 1 X TE, 1 X PA for 3-5hr with one change of solution. Melt agarose at 65°C for 10 minutes, transfer
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