010-1270赛默飞50ml 尖底管适配器，2个/套The

Stripping Western Blots

of antibody per reaction. 5. Add protein A sepharose or protein G sepharose depending on which antibody used to IP. 6. Wash complexes: 1: 2X with 0.5 ml of RIPA buffer (50 MM Tris pH 7.5, 150 mM NaCl, 1% triton-X 100, 0.1% SDS, 1% sodium