03613赛默飞离心机附件（离心管）Thermo Fishe

Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞

).remove and save polylysine.wash plates 2x with 10ml sterile water.Seed cells: 1/2 X split from confluent plate (approximately 2-5 x 106 cells per 100mm plate) in PC12 culture media.Incubate overnight in 10% CO2 incubator.Cells should be 50-70% confluent

How to build a BAC library

. Mix by slowly stirring contents. Incubate overnight at 16°C. After ligation, carry out drop-dialysis of sample against approximately 25 ml 0.5 X TE, 1 X PA for 2 hours at room temperature in a 100 mm petri dish. 1 X PA is a mixture of spermine

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65