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文献和实验can be increased to 75 mM in the Elution buffer if final DNA yields are consistently low). 5. Add 0.6 ml of Elution buffer and collect the flow-through in sterile eppendorf tubes placed in a Sarstedt rack. Transfer a 10 µl aliquot into another eppendorf tube
(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP
Procedures for In Vitro Production of Bovine Embryos
OF FERTILIZED OOCYTES INTO EMBRYO CULTURE DROPS (8-10 h post IVF)Materials and Equipment Needed Vortexer Timer 1.5 ml Dolphin microcentrifuge tube and rack X-plate (with prewarmed HEPES-TALP) Plastic Pasteur pipet Heater (placed in front of the microscope) Slide
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