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文献和实验of BSTX1 Adapters Note:Thaw adaptors on ice--be careful to never warm adapters. Speed vac your 30µl DNA sample down to 23µl. To setup the ligation of BAC insert to adapters, add the following components to a 1.5ml microfuge tube. Set up with all tubes
of minimum area. In a fixed angle rotor, the materials are forced against the side of the centrifuge tube, and then slide down the wall of the tube. This action is the primary reason for their apparent faster separation, but also leads to abrasion
as horizontal rotors) have the advantage that there is usually a clean meniscus of minimum area. In a fixed angle rotor, the materials are forced against the side of the centrifuge tube, and then slide down the wall of the tube. This action is the primary reason
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