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文献和实验Stainless steel mesh screen (200 mesh=74 mm,Type 316; Tylinter,Mentor,Ohio) RPMI-1640 medium Triton X-100 Lysis Buffer: 50 mM Tris-HCl,pH 7.5 150 mM NaCl 0.02% NaN3 10 mg/ml Aprotinin 10 mg/ml Pepstatin 10 mg/ml Leupeptin 1 mM PMSF (Made fresh
Purpse The prtcl describes hw t prepare human tnsil lysate fr use in purificatin f ICAM-1,LFA-1,and PNAd. Materials Safety Equipments Lab Cat Latex Glves Face Mask Bench Paper Fresh human tnsil tissue Parafilm Scissrs Stainless steel
in purification of ICAM-1,LFA-1,and PNAd. Materials Safety Equipments Lab Coat Latex Gloves Face Mask Bench Paper Fresh human tonsil tissue Parafilm Scissors Stainless steel mesh
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