75003812赛默飞离心机附件Thermo Fisher

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

Shotgun Library

of BSTX1 Adapters Note:Thaw adaptors on ice--be careful to never warm adapters. Speed vac your 30µl DNA sample down to 23µl. To setup the ligation of BAC insert to adapters, add the following components to a 1.5ml microfuge tube. Set up with all tubes

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2