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- 2025年12月31日
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文献和实验Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
5‐ to 15‐ml round‐bottom high‐speed (40,000 × g ) centrifuge tubes (e.g., Fisher) Polytron tissue homogenizer (Brinkmann) or Tissue Tearor (Fisher
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
in culture. (B ) Neurosphere attachment to a poly-D-lysine/laminin-coated slide, 1 d after plating. (C ) β-tubulin-III (red) and glial fibrillary acidic protein (GFAP) (green) labeling of neurosphere-derived neurons and astrocytes, respectively
to 1 liter store at 4oC Valap* Put 50 g Vaseline, 50 g lanolin, and 50 g paraffin (all available from Fisher) in a 1 L Pyrex beaker Heat on "low" setting on a hotplate, stirring occasionally, until all components are melted and well mixed Pour
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