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- Thermo Fisher
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- 2025年12月31日
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文献和实验Calcium Phosphate Transfection of PC12 Cells 磷酸钙法转染PC12细胞
(one bottle) in 250 ml sterile H2O (tf 20ug/ml) store at 4C, re-use for a few months.PC12 culture media DMEM (lab home-made) 10% Horse serum 5% Fetal Bovine Serum (calf serum may also be fine) 2mM glutamine (1/100 from lab stock) penn/strep (1/200
2. PEG/DME: autoclave 10 g PEG (PEG1500, BDH limited, 29575) in a 100 ml medium bottle, then add immediately 10 ml warm (37o C) DME (no additive) and mix by swirling until the solution is homogeneous. Warm up to 37o before use. Store at room temperature
It consists of 250 mM glucose in H2 O and can be stored in aliquots at −20 °C. Internalization medium The internalization medium consists of 1 g D -glucose in one bottle (500 ml) OptiMEM and is stored
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