88881138赛默飞40x18mm可调节试管架Thermo

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

In Situ HYBRIDIZATION TO TISSUE SECTIONS

ml glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108

Immunostaining of Paraffin Sections

around sections, then add one or two drops of secondary peroxidase-labeled antibody diluted 1/1,000 in PBS containing 1 BSA.  Incubate for 60 min at room temp on staining rack.  Wash five times over 40 min in PBS using squirt bottle.