03278赛默飞离心机附件（离心管）Thermo Fishe

【转贴】immunofluorescence staining

Scientific, Cat. #6663-F10, #1 thickness 11 X 22 mm rectangular coverslips.22 X 22 mm coverslips may also be used, but will require larger volumes of reagents:Fisher Scientific, Cat. #12-520-B, #1 1/2 thickness 22 X 22 mm square coverslips.Note

A quick RNA mini-prep for Neurospora mycelial cultures

with phenol/chloroform/isoamyl alcohol (25:24:1), spinning 12000g 10 min at 4°C in a fixed angle rotor. Save aqueous (upper) phase; add gd H2 O if volume is less than 2 ml. 12. Add 0.1 vol 3M NaOAc pH 6.0, mix and add 2.5 vol EtOH, mix. Place at -20°C

质谱的试剂用品、实验操作、仪器和样品选择方法

(Fisher M/4056/17)、Acetonitrile(Fisher A/0626/17)、Trypsin (Promega V528)、Trypsin resolve solution(Promega V530)、Ammonium bicarbonate(Sigma A6141)、ZipTip μC-18(Millipore ZTC18M096)、TFA(GE HealthCare)、0.2ml and 0.5ml EP tube(eppendorf)、50ml EP 0.5ml EP