88870104赛默飞28 x 2.0ml离心管加热块，适用

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

Immunohistochemistry: Fluorescence Protocol 2.0

. Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x g for 5 minutes) at 4°C to remove extracellular cytokines. Add 0.5 mL of the Fixation Buffer

DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)

at 65°C for 30 minutes to re-hydrate the DNA. Protein Isolation: 26. Take the tube contains protein pellet from step 7. 27. Add 1ml of 95% ethanol and vortex the tube for 30 seconds. 28. Centrifuge at maximum speed for 2 minutes