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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
at 3000g, 4°C in a fixed angle rotor. Make sure caps are screwed on tightly to prevent tubes from collapsing. 8. Pass supernatant through 50 micron Miracloth (Calbiochem #475855, La Jolla, CA) in a funnel into fresh Sarstedt tube. 9. Measure
up too rapidly and break. The trick is to gently dry the smear without overheating the slide. 5. Place the slide on a staining rack. Apply the stains on the fixed smear as follows: Flood the slide with crystal violet for 30 sec. Rinse with water
(medicine spoon)×1 (Erlenmeyer flask) 100 ml×3 滴管(drip tube;dropper)×2 烧杯(beaker) 500 ml×1 玻棒(Glass stic)×2 250 ml×3 木试管夹(test tube clamp;test tube holder)×1 100 ml×3 胖肚吸管(straws) 25 ml×1 50 ml×2 10 ml×1 容量瓶(volumetric flask) 100 ml×2 乳钵(morta)×
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