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文献和实验Clonality - X Chromosome Inactivation Assay
This method was successful in our lab using prostate tissue and for our specific objectives. Investigators must be aware that they will need to tailor the following protocol for their own research objectives and tissue under study. Investigators
Northern Blotting方法(Howell Lab)
buffer. Pour GITC/Cell mix into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes
We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
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