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文献和实验Northern Blotting方法(Howell Lab)
X MOPS to one liter. Sample preparation To 0.5 ml Eppis, add: a) 15 µg RNA (approxiamately 4.5 µl) b) 14.5 µl sample buffer c) Adjust with DEPC water so that final volumes are equal d) Heat for 5 min at 68℃, then chill
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
. However, you may have to optimize conditions for your specific tissue type.Day 11. Chop tissue into small pieces with a razor blade or scalpel. Transfer tissue into a tube with a screw cap lid and add a small (5-10mls) amount of tissue culture media. We have not noticed
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab) This protocol is based upon protocols from Mark
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