88881116赛默飞1000ml输液瓶夹具Thermo F

小RNA的Northern BLOT

material, swirl bottle often.  Take out ~25 ml (1ml/10cm2) in 50 ml conical tube, preheat @ 37℃.  Add hyb solution to hyb bottle and pre-hyb for at least 5 min. Pre-hyb:___________ Hybridization:  Pour out ~10 ml of pre-hyb

SDS-PAGE

. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue

Methods for DNA isolation

the cell pellets in a total of 70 ml of GET/Lysozyme solution (35 ml for each bottle) by gently teasing the pellet with a spatula and incubate for 10 minutes at room temperature. (Note: Do not vortex the lysate at any time because this may shear