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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- 规格:
100ug; 500ug; 1mg; Bulk

| 产品信息
品名:NebuSelect™ Recombinant Human IGF1, 49-118 aa, Tag free
中文名称:重组人IGF1蛋白
别名:IGF1, IGF-I, IGF1, Mechano growth factor, MGF, Insulin-like growth factor I, IBP1, Somatomedin-C
货号:NBL-288065
品牌:Nebulabio
规格:100UG;1MG;Bulk
| 产品描述
NebuSelect™ Recombinant Human IGF1, 49-118 aa, Tag free (Cat# NBL-288065) is expressed in Yeast with Tag free. This recombinant protein is of high purity, verified to be greater than 90% by SDS-PAGE. Endotoxin levels are less than 1 EU/µg, as determined by the LAL (Limulus Amebocyte Lysate) assay. Please contact us freely to get bioactivity data.
| 产品属性
Species:Human
Expression System:Yeast
Molecular Weight:7 kDa
Target Name:IGF1
Purity:>90%
Formulation:See COA
Endotoxin:<1 EU/ug(LAL Method)
Storage:4°C for short term. -20°C for long term.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验Differentiating Neural Stem Cells into Neurons and Glial Cells
Medium Antibiotic-Antimycotic solution Fetal Bovine Serum, ES Cell-Qualified FBS GlutaMAX™-I FGF-basic (AA 10–155), Recombinant Human (bFGF) EGF, Recombinant Human CELLstart™ CTS™ Geltrex™ Reduced Growth Factor
Strategies to Optimize Protein Expression in E. coli
., Wang, Y., Ma, J.B., Gao, D.M., Wu, H.M., and Chen, H.B. 2006. Over‐expression and purification of isotopically labeled recombinant ligand‐binding domain of orphan nuclear receptor human B1‐binding factor/human liver receptor homologue 1 for NMR studies
之类的,所以不知如何鉴定它的活性。 如果我得到的是变性的融合蛋白,那么用于制备多抗或者单抗会有影响吗? 如果我的融合蛋白是可溶性的,那么用GSH sepharose 4B纯化得到的融合蛋白可以直接用于制备多抗吗?溶液中的GSH,Tis等成分对免疫动物有影响吗?是否需要透析除取这些成分才能去免疫动物呢? 答:1)。不可以。GST 具体多大忘记了,但做为TAG 使用的蛋白一般是很小的一段AA,可以用相对应的抗体做PULL DOWN,亲和纯化等。但蛋白的活性是需要构象存在的,一小段TAG 空间
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