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- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Human T273A Protein
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:(CP-H)(Protein II)
应用笔记:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
纯度:Greater than 85% as determined by SDS-PAGE.
生物来源:Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
蛋白序列:CEWDEAATALEINLEEEDDDNEDEVDEQAEQQKTHVFGQAPYSGINITKEGIQIGVEGQTPKYADKTFQPEPQIGESQWYETEINHAAGRVLKKTTPMKPCYGSYAKPTNENGGQGILVKQQNGKLESQVEMQFFSTTEAAAGNGDNLTPKVVLYSEDVDIETPDTHISYMPTIKEGNSRELMGQQSMPNRP
分子量:28.8 kDa
表达区域:133-324aa(T273A)
标签:N-terminal 10xHis-tagged and C-terminal Myc-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:E.coli
形态:Liquid or Lyophilized powder
Uniprot ID:P04133
Note:For research use only.

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文献和实验Techniques for generating human T-cell clones (TCCs) were first described nearly two decades ago (1 , 2 ). This was a direct consequence of the discovery of T-cell growth factor and the subsequent ability to propagate T-cells over extended
Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
Generation of Human T Cell Clones
in several experimental settings. The availability of a population of human T lymphocytes deriving from the same progenitor with a unique phenotype and function (clone) may therefore be of help. This chapter describes the basic procedures that can be used
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