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- 详细信息
- 技术资料
- 保存条件:
Store in working aliquots at -70˚°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
- 保质期:
6个月
- 英文名:
SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein
- 库存:
详询客服
- 供应商:
biorbyt
- 规格:
0.05 mg
产品描述:SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) Recombinant Protein
产品别名:S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, Delta variant spike RBD
验证过的应用:ELISA, SDS-PAGE, WB
应用笔记:This recombinant protein can be used for WB and ELISA. For research use only.
纯度:> 95% as determined by Bis-Tris PAGE
蛋白序列:Arg319-Phe541 (T478K, L452R)-His-Avi
分子量:35 kD
标签:His-Avi tag at C-terminus
防腐剂:PBS (pH 7.4).
来源:HEK293 cells
形态:Liquid
NCBI:QHD43416.1
Uniprot ID:P0DTC1
Note:For research use only.






产品别名:S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, Delta variant spike RBD
验证过的应用:ELISA, SDS-PAGE, WB
应用笔记:This recombinant protein can be used for WB and ELISA. For research use only.
纯度:> 95% as determined by Bis-Tris PAGE
蛋白序列:Arg319-Phe541 (T478K, L452R)-His-Avi
分子量:35 kD
标签:His-Avi tag at C-terminus
防腐剂:PBS (pH 7.4).
来源:HEK293 cells
形态:Liquid
NCBI:QHD43416.1
Uniprot ID:P0DTC1
Note:For research use only.

ELISA Binding Assay of ACE2 and SARS-CoV-2 (COVID-19) Delta Variant Spike RBD Recombinant Protein, Coating Antigen: SARS-CoV-2 Delta variant (B.1.671.2) spike RBD protein, 1 μg/mL, incubated at 4 °C overnight. Detection Antibodies: ACE2 recombinant protein, orb1231604, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-human HRP at 1:10, 000, incubate at RT for 1 hr.

SDS-PAGE Validation of SARS-CoV-2 (COVID-19) Delta Variant Spike RBD Recombinant Protein. Loading: 1 μg SARS-CoV-2 (COVID-19) Delta variant Spike RBD recombinant protein, orb1228207. Observed: 35kD

ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies. Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, orb1239036, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant RBD protein (orb1228207), but not WT spike RBD protein (orb1231588), was specifically detected by Delta Variant spike L452R antibody (orb1239036).

WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Delta Variant Spike L452R Antibodies. Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Delta variant spike L452R antibody, orb1239036, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. SARS-CoV-2 Delta Variant RBD protein (orb1228207), but not WT spike RBD protein (orb1231588), was specifically detected by Delta Variant spike L452R antibody (orb1239036).

ELISA Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies. Coating Antigen: SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2), 5 μg/mL, incubated at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike RBD antibody, orb1239994, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr. Antibody orb1239994 does not distinguish between WT and Delta.

WB Validation of Delta Variant Spike RBD Protein with SARS-CoV-2 Spike RBD Antibodies. Loading: 20 ng of SARS-CoV-2 spike RBD proteins, including WT and Delta variant (B.1.671.2). Detection Antibodies: SARS-CoV-2 Spike RBD antibody, orb1239994, 1 μg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20, 000, incubate at RT for 1 hr.
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SARS-CoV-2 (COVID-19) Delta Variant Spike RBD (His-Avi Tag) 重组蛋白,orb1228207,biorbyt
¥6370










