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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
6个月
- 英文名:
Human TNF protein
- 库存:
99
- 供应商:
biorbyt
- 规格:
20 ug
产品别名:Cachectin, TNF-alphaTumor necrosis factor ligand superfamily member 2 , TNF-a
应用笔记:Tag Info: N-terminal 6xHis-taggedExpression Region: 77-233Sequence Info: Partial
纯度:Greater than 90% as determined by SDS-PAGE.
生物来源:Homo sapiens (Human)
蛋白序列:VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
分子量:21.4 kDa
表达区域:77-233
标签:N-terminal 6xHis-tagged
防腐剂:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源:E.coli
形态:Liquid or Lyophilized powder
Uniprot ID:P01375
Note:For research use only.

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文献和实验Production and Characterization of Recombinant Human and Murine TNF
takes about 2 wk. Human and murine TNF can be purified from crude extracts with high yields (>50 mg from 1 L of fermentation) by hydrophobic-interaction chromatography, ion-exchange chromatography, and gel-filtration chromatography. The purity
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted
TNF in Experimental Diseases of the CNS
Tumor necrosis factor α (TNFα) is a crucial mediator involved in the communications between immune and nervous systems in physiological conditions, and its relevance is amplified during disease. Considered originally detrimental and a target
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