- ￥5590
- biorbyt
- 英国
- orb418758
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期：
6个月
- 英文名：
E. coli EDA protein
- 库存：
99
- 供应商：
biorbyt
- 规格：
20 ug
产品别名：2-keto-4-hydroxyglutarate aldolase , KHG-aldolase2-dehydro-3-deoxy-phosphogluconate aldolase (EC, 4.1.2.14) , 2-keto-3-deoxy-6-phosphogluconate aldolase , KDPG-aldolasePhospho-2-dehydro-3-deoxygluconate aldolasePhospho-2-keto-3-deoxygluconate aldolase
应用笔记：Tag Info: N-terminal 6xHis-taggedExpression Region: 1-213aaSequence Info: Partial
纯度：Greater than 90% as determined by SDS-PAGE.
生物来源：Escherichia coli (strain K12)
蛋白序列：MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL
分子量：26.3 kDa
表达区域：1-213aa
标签：N-terminal 6xHis-tagged
防腐剂：If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
来源：E.coli
形态：Liquid or Lyophilized powder
Uniprot ID：P0A955
Note：For research use only.
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文献和实验Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
In recent years several techniques have been described for the introduction of DNA molecules into Escherichia coli . These are based on the findings of Mandel and Higa (1 ), who demonstrated that incubation of cells with naked bacteriophage DNA
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
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