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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
2年
- 英文名:
Recombinant RNF40 Protein
- 库存:
999
- 供应商:
允麦生物
- 规格:
100ug
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
, and so on. If the DNA molecule has promoter elements (to allow RNA polymerases to transcribe the DNA), one can also obtain usable amounts of pure RNA, and RNA with a coding region can be translated in vitro or in vivo to express a protein of interest. The PCR
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
to sample ratio of 40:1.Dialyze 4 times for four hours each time into decreasing amounts of Urea: 4M,then 2M,then 0.75M,and then 0M Urea.Spin renatured protein in the SS34 rotor for 10 min at 10K rpm.If a large pellet is detected,analyze the samples by SDS
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