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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20
- 保质期:
2年
- 英文名:
Recombinant RNF34 Protein
- 库存:
999
- 供应商:
允麦生物
- 规格:
100ug
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文献和实验Purification of recombinant sBRF M166L
, then 0.75M, and then 0M Urea. Spin renatured protein in the SS34 rotor for 10 min at 10K rpm. If a large pellet is detected, analyze the samples by SDS PAGE before proceeding with the purification to confirm that the renatured BRF is soluble. BRF
Purification of recombinant sBRF M166L
to sample ratio of 40:1.Dialyze 4 times for four hours each time into decreasing amounts of Urea: 4M,then 2M,then 0.75M,and then 0M Urea.Spin renatured protein in the SS34 rotor for 10 min at 10K rpm.If a large pellet is detected,analyze the samples by SDS
Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli
and expressed in E. coli BL21 RIS cells. Cells growing exponentially in nutrient-rich liquid medium are induced to express the recombinant protein by addition of 50 mM isopropyl β-d -1-thiogalactopyranoside for 3–6 h. After bacterial cells collected
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