- ￥2080
- YUNMAI-BIO
- 国产/进口
- YM-22340P
- 2025年12月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20
- 保质期：
2年
- 英文名：
Recombinant LCMV RNA-directed RNA polymerase L Protein
- 库存：
999
- 供应商：
允麦生物
- 规格：
100ug
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文献和实验Reverse Transcription of RNA- 20l rxn
0.01l is the 1st strand derived from 0.00075 ug of Poly A+ RNA which is the equivalent of 0.15ug of total RNA (X-H''s method works out to use the eqivalent of 0.12ug of total RNA in the RT-PCR) Controls should include: 1) primers alone
Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
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